Human Immunodeficiency Virus Type 1 Infection of Human Monocytes and Macrophages Does Not Alter Their Ability to Generate an Oxidative Burst
Identifieur interne : 001E78 ( Main/Exploration ); précédent : 001E77; suivant : 001E79Human Immunodeficiency Virus Type 1 Infection of Human Monocytes and Macrophages Does Not Alter Their Ability to Generate an Oxidative Burst
Auteurs : Carol S. Dukes [États-Unis] ; Thomas J. Matthews [États-Unis] ; J. Brice Weinberg [États-Unis]Source :
- Journal of Infectious Diseases [ 0022-1899 ] ; 1993.
Abstract
Human immunodeficiency virus type 1 (HIV-1) infects mononuclear phagocytes, cells that may serve as a reservoir for viral persistence. Infection with HIV-1 leads to progressive compromise of the immune system, resulting in infections with opportunistic pathogens and eventual death. Experiments were designed to determine if in vitro HIV-1 infection of mononuclear phagocytes would diminish their oxidative capabilities, thus decreasing their antimicrobial effectiveness. Blood monocytes and peritoneal macrophages were obtained from uninfected donors and inoculated with a monocytotropic strain of HIV-1. Hydrogen peroxide production and reduction of nitroblue tetrazolium were measured after acute stimulation of cells with PMA or a phagocytic stimulus. Despite vigorous virus production, no difference was seen in oxidative burst between uninfected cells and infected cells or between monocyte-derived and peritoneal macrophages. In conclusion, reduced antimicrobial activity of HIV-infected mononuclear phagocytes is probably not secondary to decreased ability to generate reactive oxygen species.
Url:
DOI: 10.1093/infdis/168.2.459
Affiliations:
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Dukes</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>Department of Medicine, Divisions of Infectious Diseases and International Health and of Hematology and Oncology, and Department of Surgery, Division ofGeneral and Thoracic Surgery, Duke University Medical Center, and Medical Research Service, VA Medical Center, Durham</wicri:cityArea></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>Reprints or correspondence: Dr. Carol S. Dukes, Division of Infectious Diseases (IIIH), 508 Fulton St., Durham</wicri:cityArea></affiliation></author><author><name sortKey="Matthews, Thomas J" sort="Matthews, Thomas J" uniqKey="Matthews T" first="Thomas J." last="Matthews">Thomas J. Matthews</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>Department of Medicine, Divisions of Infectious Diseases and International Health and of Hematology and Oncology, and Department of Surgery, Division ofGeneral and Thoracic Surgery, Duke University Medical Center, and Medical Research Service, VA Medical Center, Durham</wicri:cityArea></affiliation></author><author><name sortKey="Weinberg, J Brice" sort="Weinberg, J Brice" uniqKey="Weinberg J" first="J. Brice" last="Weinberg">J. Brice Weinberg</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>Department of Medicine, Divisions of Infectious Diseases and International Health and of Hematology and Oncology, and Department of Surgery, Division ofGeneral and Thoracic Surgery, Duke University Medical Center, and Medical Research Service, VA Medical Center, Durham</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Infectious Diseases</title><title level="j" type="abbrev">J Infect Dis.</title><idno type="ISSN">0022-1899</idno><idno type="eISSN">1537-6613</idno><imprint><publisher>The University of Chicago Press</publisher><date when="1993-08">1993</date><biblScope unit="vol">168</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="459">459</biblScope><biblScope unit="page" to="462">462</biblScope></imprint><idno type="ISSN">0022-1899</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-1899</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">Human immunodeficiency virus type 1 (HIV-1) infects mononuclear phagocytes, cells that may serve as a reservoir for viral persistence. Infection with HIV-1 leads to progressive compromise of the immune system, resulting in infections with opportunistic pathogens and eventual death. Experiments were designed to determine if in vitro HIV-1 infection of mononuclear phagocytes would diminish their oxidative capabilities, thus decreasing their antimicrobial effectiveness. Blood monocytes and peritoneal macrophages were obtained from uninfected donors and inoculated with a monocytotropic strain of HIV-1. Hydrogen peroxide production and reduction of nitroblue tetrazolium were measured after acute stimulation of cells with PMA or a phagocytic stimulus. Despite vigorous virus production, no difference was seen in oxidative burst between uninfected cells and infected cells or between monocyte-derived and peritoneal macrophages. In conclusion, reduced antimicrobial activity of HIV-infected mononuclear phagocytes is probably not secondary to decreased ability to generate reactive oxygen species.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Caroline du Nord</li></region></list><tree><country name="États-Unis"><region name="Caroline du Nord"><name sortKey="Dukes, Carol S" sort="Dukes, Carol S" uniqKey="Dukes C" first="Carol S." last="Dukes">Carol S. Dukes</name></region><name sortKey="Dukes, Carol S" sort="Dukes, Carol S" uniqKey="Dukes C" first="Carol S." last="Dukes">Carol S. Dukes</name><name sortKey="Matthews, Thomas J" sort="Matthews, Thomas J" uniqKey="Matthews T" first="Thomas J." last="Matthews">Thomas J. Matthews</name><name sortKey="Weinberg, J Brice" sort="Weinberg, J Brice" uniqKey="Weinberg J" first="J. Brice" last="Weinberg">J. Brice Weinberg</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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